Oxygen quenching of sensitized terbium luminescence in complexes of terbium with small organic ligands and proteins.
نویسندگان
چکیده
منابع مشابه
Development and Validation of a Terbium-Sensitized Luminescence Analytical Method for Deferiprone
A sensitive fluorometric method for the determination of deferiprone (DFP) based on the formation of a luminescent complex with Tb3+ ions in aqueous solutions is reported. The maximum excitation and emission wavelengths were 295 and 545 nm, respectively. The effects of various factors on the luminescence intensity of the system were investigated and optimized, then under the optimum conditions,...
متن کاملDetection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes.
We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities.
متن کاملDevelopment and Validation of a Terbium-Sensitized Luminescence Analytical Method for Deferiprone
A sensitive fluorometric method for the determination of deferiprone (DFP) based on the formation of a luminescent complex with Tb3+ ions in aqueous solutions is reported. The maximum excitation and emission wavelengths were 295 and 545 nm, respectively. The effects of various factors on the luminescence intensity of the system were investigated and optimized, then under the optimum conditions,...
متن کاملTerbium Luminescence from Complexes of Angiotensin II, Small Peptides, and Amino Acids
Lanthanide ions have been successfully used as luminescent probes in several biological systems. The terbium (Tb) luminescence is greatly enhanced in Tb(III)-protein systems by non-radiative energy transfer from aromatic chromophores to bound Tb ions. In this study, the luminescent properties of terbium have been used to monitor the metal-peptide interactions of angiotensin II, a linear octopep...
متن کاملSensitized terbium(III) macrocyclic-phthalimide complexes as luminescent pH switches.
Four new macrocyclic-phthalimide ligands were synthesised via the coupling of N-(3-bromopropyl)phthalimide either to cyclen (1,4,7,10-tetraazacyclododecane) itself or its carboxylate-functionalized analogues, and photophysical studies were carried out on their corresponding Tb(III) complexes in aqueous media as a function of pH. Luminescence intensities of Tb·L1a–Tb·L3a were in ‘switched off’ m...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)32585-7